久久久一区二区四区,女性性高潮国产成人影视,欧美亚洲国产精品久久第一页,亚洲精品aⅴ中文字幕乱码麻豆

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠正常細(xì)胞>CRL-1772C2C12 小鼠成肌細(xì)胞

C2C12 小鼠成肌細(xì)胞

簡要描述:CRL-1772 C2C12 小鼠成肌細(xì)胞,
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|;
細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,
提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-1772
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-04-26
  • 訪  問  量:2388

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1772 C2C12 小鼠成肌細(xì)胞的詳細(xì)介紹

CRL-1772 C2C12 小鼠成肌細(xì)胞

ATCC® Number:CRL-1772™  Price:$256.00
Designations:C2C12

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:myoblast
C2C12 小鼠成肌細(xì)胞


Source:Strain: C3H
Tissue: muscle
Cell Type: myoblast;


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this . AATCC materialnyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-1772

Applications:transfection host (Nucleofection technology from Lonza

Roche FuGENE® Transfection Reagents)



Comments:This is a subclone (produced by H. Blau, et al) of the mouse myoblast cell line established by D. Yaffe and O. Saxel. [22903]

The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins. [22953]

Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic. [23427]

Tested and found negative for ectromelia virus (mousepox).



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C


Subculturing:Protocol: IMPORTANT - DO NOT ALLOW CULTURES TO BECOME CONFLUENT.

Cultures must not be allowed to become confluent as this will deplete the myoblastic population in the culture.

Myotube formation is enhanced when the medium is supplemented with 10% horse serum instead of fetal bovine serum.

  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

    Inoculate at a cell concentration between 1.5 X 10 exp5 and 1.0 X 10 exp6 viable cells/75 cm2.

  6. Incubate cultures at 37°C.





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产精品免费99久久久| 欧美日韩综合在线一区| 丁香婷婷亚洲六月综合色| 翘臀小穴在线观看| 亚洲国产成人久久成人52| 国产一区二区三区在线观| 澳门一区二区免费下线观看| 在线无码一区二区三区不卡| 亚洲欧美日韩中文v在线| 精品国产99亚洲一区二区三区| 日本亚洲欧洲一区二区| 三级成人国产高清视频| 男人大鸡巴操女人的大逼| 正在播放 国产精品推荐| 日本福利一区二区视频| 久久久精品日韩一区二区三区| 日韩久久奶茶视频| 使劲操大骚逼av| 黄网官方在线观看| 久久综合娱乐中文网| 日韩在线中文字幕在线视频| 欧美一区二区三区四公司| 亚洲精品一区二区精华液| 精华欧美一区二区久久久| 欧美激情在线播放第一页| 黄色录像片操大逼的| 思思夜免费公开视频| 最新AV中文字幕在线看| 亚洲卡通动漫第127页| 老司机免费福利午夜入口| 人妻在线系列一区二区三| 男生的小鸡鸡插进女生的桃子 里| 欧美老熟妇又粗又大| 插逼爽歪歪视频免费| 夜夜嗨av少妇一二三区| 欧美 日韩 激情 在线| 国产精品毛片一区视频播| 手机成人三级a在线观看| 鸡巴操美女小穴羞羞视频| av人摸人人人澡人人超| 伊人久久亚洲婷婷综合久久|