久久久一区二区四区,女性性高潮国产成人影视,欧美亚洲国产精品久久第一页,亚洲精品aⅴ中文字幕乱码麻豆

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>COLO-320人結(jié)腸腺癌細(xì)胞

人結(jié)腸腺癌細(xì)胞

簡要描述:COLO-320 人結(jié)腸腺癌細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;
細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,
提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):COLO-320
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-04-25
  • 訪  問  量:2193

產(chǎn)品分類

Product Category

詳細(xì)介紹

COLO-320 人結(jié)腸腺癌細(xì)胞

ATCC® Number: CCL-220™

Designations: COLO 320DM

Depositors: GE Moore

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed, adherent and suspension

Organism: Homo sapiens (human)

Morphology: rounded and refractile

COLO-320 人結(jié)腸腺癌細(xì)胞

Source: Organ: colon

Tumor Stage: Dukes' type C

Disease: colorectal adenocarcinoma

Cellular Products: serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Tumorigenic: Yes

Reverse Transcript: negative

DNA Profile (STR): Amelogenin: X

CSF1PO: 11

D13S317: 11

D16S539: 11,12

D5S818: 12

D7S820: 9,12

THO1: 9

TPOX: 8,9

vWA: 15,18

Cytogenetic Analysis: modal number = 24; range = 49 to 61.

The stemline chromosome number is hyperdiploid and 9-11 marker chromosomes (M1, M2, M3, M5, M10, M11, M12, M13, M14, 7p- and 10 q+) were common. Double minutes were observed in 24% of the metaphases examined.

Isoenzymes: ES-D, 1

G6PD, B

PEP-D, 1

PGD, A

PGM1, 1

PGM3, 2

Age: 55 years

Gender: female

Ethnicity: Caucasian

Comments: The line is negative for CEA, CSAp and colon antigen 3.

The cells are weakly positive for keratins and vimentin.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Shake flask, pour one-half of the medium into a new flask and add fresh medium to both flasks. Cells remaining attached may be removed (if desired) using a standard trypsin protocol.

Add 2.0 to 3.0 of 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:5 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO

Storage temperature: Liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 1045: Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117

22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products

COLO-320



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
粉色av一区二区三区| 无码社区在线观看| 黄色软件大屌怒戳粉嫩小穴| 国奴精品毛片av一区二区三区| 国产大码丝袜老熟女av| 国产精品三二一免费| 外国处女BB视频| 欧美日韩国产欧美| 美国业余自由摘花管| 天堂久久久久久久久久久| 亚洲欧美国产原创一区二区三区| 中文字幕在线观视频| 黑丝美女被操到高潮| 欧美黑屌操B内射冒白浆| 日国产精品1卡二卡三卡| 欧美高清一二三区| 男女真人牲交高潮全过程| 国产高清免费一级a久久| 国产精品538一区二区在线| 女人逼逼出水视频| 国产欧美日韩一区二区在线观看| 国产亚洲一区白丝在线观看| 阴茎大头插少妇蜜穴视频| 亚洲国产成人手机版| 大屁股真人日逼视频| 一个色综合色综合色综合| 国产精品久久大屁股白浆| 国产乱精品一区二区三区视频了| 日韩免费一级a毛片在线播放一级| 国产欧美日韩一区二区在线观看| 中文有码无码人妻在线看| 我和两个老师的浮乱生活| 老司机精品成人无码AV| 欧美精品国产一区二区在线观看| 把女生操出水的视频| 国产一区二区三区三级88| 久久精品男人的天堂av| 久久久久精品无码专区喝奶| 日本精品高清在线观看| A级毛片毛片免费观看久| 女教师色色天天免费播放|